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Gupta, A.
- Improved Production of L-Lysine by Immobilized Biotin Auxotropic Mutant Micrococcus glutamicus AB200
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Authors
Affiliations
1 Department of Chemical Engineering, University of Calcutta, Kolkata – 700 009
1 Department of Chemical Engineering, University of Calcutta, Kolkata – 700 009
Source
Asian Journal of Research in Chemistry, Vol 6, No 7 (2013), Pagination: 613-617Abstract
Experimental studies were carried out to examine the efficiency of whole cell immobilization in calcium alginate beads and agar blocks for the production of L-lysine using a biotin autotrophic mutant Micrococcus glutamicus AB<SUB>200</SUB> developed in laboratory by induced mutation from a regulatory mutant Micrococcus glutamicus AB5. Under the same physico- chemical environment, using selected suitable synthetic medium, different parameters for both calcium alginate beads and agar blocks were optimized one by one to maximize the production of L-lysine. Production was increased significantly with cells entrapped in calcium alginate beads compared to free cells with the presence of 0.2(M) CaCl<SUB>2</SUB> in synthetic medium, 0.2 (M) CaCl<SUB>2</SUB> for bead formation, 4% sodium alginate, 24 h storage period of beads and 1.5 cell/alginate ratio but the accumulation of was decreased significantly with the whole cell entrapped in agar blocks, compared to free cells.Keywords
Immobilization, L-lysine, Calcium Alginate Bead, Encapsulation, Mutant
References
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- Solid Phase Synthesis and Their Screening System-Review
Abstract Views :216 |
PDF Views:1
Authors
Affiliations
1 National Institute of Ayurvedic Pharmaceutical Research, CCRAS, Dept. of Ayush, Patiala, IN
2 National Botanical Research Institute, Rana Pratap Marg, Lucknow, IN
1 National Institute of Ayurvedic Pharmaceutical Research, CCRAS, Dept. of Ayush, Patiala, IN
2 National Botanical Research Institute, Rana Pratap Marg, Lucknow, IN
Source
Asian Journal of Research in Chemistry, Vol 4, No 3 (2011), Pagination: 362-369Abstract
The use of solid phase synthesis techniques has been explored as an alternative to conventional approaches for the synthesis of compounds used in the drug discovery process. Combinatorial chemistry now plays an important role in the lead discovery and hit optimization processes in the pharmaceutical world as well as in other areas of discovery chemistry. Now days this is an important technique for the development of different synthesis concepts that were intended to cover and explore the chemical space without having to prepare every individual compound. Solid phase synthesis technologies were developed in response to the increased screening capacities and target oriented lead finding. This article explains different linker, protecting group, cleavage methods used in solid phase synthesis, combinatorial libraries and screening system used in solid phase synthesis.